Cu/Zn recombinant hunman Superoxide DismutaseMaterial extraction method,this method will include human body SOD the gene PCR252 carrier backwoods coli to meet in the seed culture medium, the fermentation raises, joins the deionized water again to the fermentation fluid, is stave with the vibration, the centrifugal separation, except the precipitation impurity, ultra filters the concentration, joins the cold acetone again to the thick extraction fluid, the fractionation precipitation, the recentrifuging separation, except the precipitation impurity, ultra filters the concentration,The freeze-drying, namely for medicinal purposes SOD freezing and drying, this product enzyme activity is 8000U~15000U/mg, this invention uses the biological gene duplicate uniform hyperoxide mutase to have viruss hidden one kind to reorganize the person hyperoxide mutases extraction method, its characteristic lies in this method including the following step; (1) will include human body SOD the gene PCR252 carrier backwoods coli to meet in the seed culture medium, under 32 ℃ temperatures, the fermentation raises for 24 hours,Joins again occupies the fermentation fluid weight 1% cupric sulfate serum, joins 3 time of volumes again to the fermentation fluid the deionized water, with vibration type breaker strenuous vibration stave 30min, settles 20min, posts in each minute 2000 revolution of centrifuges, centrifugal separation, except precipitation impurity, limpid transparent thick enzyme fluid;(2) posts the thick enzyme fluid in the pure enzyme meter to separate, the decon, joins again 4℃ the isometric volumes deionized water, ultra filters the concentration, results in isometric volume 50% SOD the thick extraction fluid, joins the isometric 4 ℃ cold acetone again to the thick             extraction fluid, the fractionation precipitation, removes the base impurity, results in the SOD concentrated solution;(3) the SOD concentrated solution with 2 time of volumes deionized water dissolutions, posts once more in each minute 2000 revolution of centrifuges, the centrifugal separation, except the precipitation impurity, results in the SOD concentration serum, after will concentrate the serum 4℃ freezes 48 hours static, except the precipitation impurity, ultra filters the concentration, the freeze-drying,Namely for medicinal purposes SOD freezing and drying, this product enzyme activity is the 8000U~15000U/mg trouble, enables it to have the simple craft, the low processing cost, the extraction time is short and the extraction rate is high.